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一步法人胱抑素C(Cys-C)酶聯(lián)免疫吸附檢測試劑盒

ELK005ES

規格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Human Cys-C(CystatinC) ELISA Kit
Reactivity:	Human
Alternative Names:	CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type:	Sandwich
Sensitivity:	25 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.1-5000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.781	2.716
2500.00	1.701	1.637
1250.00	0.906	0.842
625.00	0.508	0.443
312.50	0.319	0.254
156.25	0.177	0.113
78.13	0.120	0.056
0.00	0.064	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.

Matrix	Recovery range	Average
serum(n=5)	88-99%	94%
EDTA plasma(n=5)	102-110%	104%
Heparin plasma(n=5)	98-104%	101%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.