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一步法大鼠脂聯(lián)素(ADP)酶聯(lián)免疫吸附檢測試劑盒

ELK030ES

規格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Rat ADP(Adiponectin) ELISA Kit
Reactivity:	Rat
Alternative Names:	GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
Assay Type:	Sandwich
Sensitivity:	4.75 ng/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Metabolic pathway;Infection immunity;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP, and the Rat ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat ADP. After TMB substrate solution is added, only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADP in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
500.00	1.908	1.811
250.00	1.609	1.512
125.00	1.194	1.097
62.50	0.971	0.874
31.25	0.456	0.359
15.63	0.321	0.224
7.82	0.216	0.119
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ADP and the recovery rates were calculated by comparing the measured value to the expected amount of ADP in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	78-93%	85%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.