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人上皮型鈣黏蛋白(E-Cad)酶聯(lián)免疫吸附檢測試劑盒

ELK1006

規格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview

Product name:	Human E-Cad(E-Cadherin) ELISA Kit
Reactivity:	Human
Alternative Names:	CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type:	Sandwich
Sensitivity:	25.1 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;
Uniprot ID:	P12830
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.201	2.076
2000.00	1.717	1.592
1000.00	1.251	1.126
500.00	0.806	0.681
250.00	0.539	0.414
125.00	0.364	0.239
62.50	0.258	0.133
0.00	0.125	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	82-95%	88%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.