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人胰島素樣生長(zhǎng)因子1(IGF1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1134

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human IGF1(Insulin Like Growth Factor 1) ELISA Kit
Reactivity:	Human
Alternative Names:	IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
Assay Type:	Sandwich
Sensitivity:	0.067 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Endocrinology;
Uniprot ID:	P05019
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Biomedicines

Comparison between Platelet Lysate, Platelet Lysate Serum, and Fetal Bovine Serum as Supplements for Cell Culture, Expansion, and Cryopreservation

日期：2024-01-02 IF：4.7
Journal of Maxillofacial and Oral Surgery

Expression of Salivary and Serum IGF-1 and IGFBP-3 in Individuals With Diabetes and Oral Cancer

日期：2024-05-24 IF：0.9
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets

日期：2024-03-25 IF：4.9
Research Square

Analysis of prevalence and associated biomarkers of sarcopenia living in rural community older adults in Wuhan, China

日期：2024-08-20 IF：3.8

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.154	2.072
5.00	1.593	1.511
2.50	1.174	1.092
1.25	0.797	0.715
0.63	0.532	0.450
0.32	0.335	0.253
0.16	0.319	0.237
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IGF1 and the recovery rates were calculated by comparing the measured value to the expected amount of IGF1 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-95%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.