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人基質(zhì)細(xì)胞衍生因子1(SDF1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1183

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human SDF1(Stromal Cell Derived Factor 1) ELISA Kit
Reactivity:	Human
Alternative Names:	CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P48061
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SDF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

WOUND REPAIR AND REGENERATION

Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway

日期：2022-04-06 IF：3.617
Biologi dan Pendidikan Biologi

Characteristics of Umbilical Cord Derived Mesenchymal Stem Cell/UCMSC from Macaca fascicularisand Its Secretome Under Hypoxic Conditions

日期：2024-06-30 IF：0.7
European Journal of Endocrinology

Concomitant gut dysbiosis and defective gut barrier serve as the bridges between hypercortisolism and chronic systemic inflammation in Cushing disease

日期：2024-10-26 IF：5.3

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
10.00	1.983	1.901
5.00	1.562	1.480
2.50	1.135	1.053
1.25	0.782	0.700
0.63	0.541	0.459
0.32	0.315	0.233
0.16	0.225	0.143
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SDF1 and the recovery rates were calculated by comparing the measured value to the expected amount of SDF1 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-105%	100%
EDTA plasma(n=5)	93-101%	97%
Heparin plasma(n=5)	85-103%	94%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SDF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.