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小鼠轉化生長(cháng)因子β1(TGFb1)酶聯(lián)免疫吸附檢測試劑盒

ELK1186

規格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Mouse TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Assay Type:	Sandwich
Sensitivity:	6.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P04202
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.204	2.116
500.00	1.782	1.694
250.00	1.069	0.981
125.00	0.863	0.775
62.50	0.549	0.461
31.25	0.342	0.254
15.63	0.227	0.139
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TGFb1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGFb1 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	95-107%	101%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFb1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.