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人激肽釋放酶5(KLK5)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1203

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human KLK5(Kallikrein 5) ELISA Kit
Reactivity:	Human
Alternative Names:	KLK-L2; KLKL2; SCTE; Kallikrein-Related Peptidase 5; Kallikrein-like protein 2; Stratum corneum tryptic enzyme
Assay Type:	Sandwich
Sensitivity:	12.5 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, saliva, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;
Uniprot ID:	Q9Y337
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLK5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Reproduction Open

Influence of ovarian stromal cells on human ovarian follicle growth in a 3D environment

日期：2023-12-21 IF：8.3

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.266	2.171
1000.00	1.563	1.468
500.00	1.121	1.026
250.00	0.975	0.880
125.00	0.509	0.414
62.50	0.236	0.141
31.25	0.213	0.118
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant KLK5 and the recovery rates were calculated by comparing the measured value to the expected amount of KLK5 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	84-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLK5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.