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睪酮(Testo)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1332

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Testo(Testosterone) ELISA Kit
Reactivity:	General
Alternative Names:	T; Testesteron; Testeronun
Assay Type:	Competitive Inhibition
Sensitivity:	17.7 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Leydig Cells in Immunocastrated Polish Landrace Pig Testis: Differentiation Status and Steroid Enzyme Expression Status

日期：2022-05-30 IF：6.208
Agriculture

Intense Leisure Exploitation Influences on Horses Hormonal Reaction—Preliminary Study

日期：2022-10-26 IF：-
Theriogenology

Senescence and autophagy relation with the expressional status of non-canonical estrogen receptors in testes and adrenals of roe deer (Capreolus capreolus) during the pre-rut period

日期：2022-12-29 IF：2.923
preprints

Risk Assessment of Transgender People: Development of Rodent Models Mimicking Gender-Affirming Hormone Therapies and Identification of Sex-Dimorphic Liver Genes as Novel Biomarkers of Sex Transition

日期：2022-11-18 IF：-
Comparative Clinical Pathology

Ameliorative effects of quercetin on endocrine and metabolic abnormalities associated with experimentally induced polycystic ovary syndrome in mice

日期：2023-02-08 IF：-
Food and Chemical Toxicology

Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis

日期：2023-05-01 IF：5.572
Frontiers in Endocrinology

Nitric oxide-induced lipophagic defects contribute to testosterone deficiency in rats with spinal cord injury

日期：2024-02-21 IF：6.7
Frontiers in Endocrinology

Nitric oxide-induced lipophagic defects contribute to testosterone deficiency in rats with spinal cord injury

日期：2024-02-22 IF：6.7
Phytomedicine

Dihydrotanshinone I targets ESR1 to induce DNA double-strand breaks and proliferation inhibition in hepatocellular carcinoma

日期：2024-07-25 IF：6.7
Stem Cell Research & Therapy

Model construction and drug therapy of primary ovarian insufficiency by ultrasound-guided injection

日期：2024-02-20 IF：7.5

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	0.183
2000.00	0.332
1000.00	0.567
500.00	0.823
250.00	1.157
125.00	1.537
62.50	1.715
0.00	2.213

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Testo and the recovery rates were calculated by comparing the measured value to the expected amount of Testo in samples.

Matrix	Recovery range	Average
serum(n=5)	90-103%	96%
EDTA plasma(n=5)	81-96%	88%
Heparin plasma(n=5)	78-94%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testo and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.