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小鼠WNT1誘導信號通道蛋白1(WISP1)酶聯(lián)免疫吸附檢測試劑盒

ELK1333

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview

Product name:	Mouse WISP1(WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein
Assay Type:	Sandwich
Sensitivity:	2.55 pg/mL
Standard:	400 pg/mL
Detection Range:	6.25-400 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	O54775
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WISP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WISP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WISP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse WISP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
400.00	2.073	1.991
200.00	1.647	1.565
100.00	1.185	1.103
50.00	0.838	0.756
25.00	0.617	0.535
12.50	0.436	0.354
6.25	0.211	0.129
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant WISP1 and the recovery rates were calculated by comparing the measured value to the expected amount of WISP1 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WISP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.