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小鼠腫瘤壞死因子受體超家族成員5(TNFRSF5)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1380

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Mouse TNFRSF5(Tumor Necrosis Factor Receptor Superfamily, Member 5) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CD40; CDW40; Bp50; P50; B-cell surface antigen CD40; CD40L receptor
Assay Type:	Sandwich
Sensitivity:	5.4 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Immune molecule;
Uniprot ID:	P27512
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFRSF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFRSF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFRSF5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Open Veterinary Journal

Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics

日期：2024-08-31 IF：0.9

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.166	2.101
500.00	1.482	1.417
250.00	1.116	1.051
125.00	0.831	0.766
62.50	0.508	0.443
31.25	0.365	0.300
15.63	0.113	0.048
0.00	0.065	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TNFRSF5 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF5 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	86-99%	92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.