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人成纖維細胞生長因子4(FGF4)酶聯(lián)免疫吸附檢測試劑盒

ELK1416

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human FGF4(Fibroblast Growth Factor 4) ELISA Kit
Reactivity:	Human
Alternative Names:	HBGF4; HST; HSTF1; KFGF; K-FGF; Heparin Secretory Transforming Protein 1; Kaposi Sarcoma Oncogene; Transforming Protein KS3; FGF-Related Oncogene
Assay Type:	Sandwich
Sensitivity:	6 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P08620
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.171
500.00	1.811	1.716
250.00	1.147	1.052
125.00	0.895	0.800
62.50	0.585	0.490
31.25	0.387	0.292
15.63	0.230	0.135
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FGF4 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF4 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	83-95%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.