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人成纖維細(xì)胞生長(zhǎng)因子6(FGF6)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1417

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human FGF6(Fibroblast Growth Factor 6) ELISA Kit
Reactivity:	Human
Alternative Names:	HBGF6; HST2; HST2 Oncogene; Heparin Secretory-Transforming Protein 2; Heparin-Binding Growth Factor 6
Assay Type:	Sandwich
Sensitivity:	3.3 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P10767
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGF6 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
500.00	2.201	2.124
250.00	1.779	1.702
125.00	1.386	1.309
62.50	0.971	0.894
31.25	0.630	0.553
15.63	0.413	0.336
7.82	0.219	0.142
0.00	0.077	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FGF6 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF6 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-107%	98%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	86-96%	91%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.