免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

大鼠B-細(xì)胞淋巴瘤因子2(Bcl2)酶聯(lián)免疫吸附檢測(cè)試劑盒
ELK1525
規(guī)格: 價(jià)格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Rat Bcl2(B-Cell Leukemia/Lymphoma 2) ELISA Kit
Reactivity: Rat
Alternative Names: B-Cell CLL/Lymphoma 2
Assay Type: Sandwich
Sensitivity: 28 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Apoptosis;
Uniprot ID: P49950
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bcl2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bcl2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL) OD Corrected OD
5000.00 2.096 2.020
2500.00 1.587 1.511
1250.00 1.142 1.066
625.00 0.855 0.779
312.50 0.576 0.500
156.25 0.297 0.221
78.13 0.134 0.058
0.00 0.076 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Bcl2 and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 82-98% 90%
Heparin plasma(n=5) 80-97% 88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 95-106% 87-101% 82-97%
EDTA plasma(n=5) 80-92% 86-102% 87-103% 85-101%
Heparin plasma(n=5) 85-94% 90-99% 95-102% 82-90%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號(hào)

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
军事| 扎赉特旗| 武汉市| 肃南| 嘉祥县| 石泉县| 濮阳市| 怀远县| 襄城县| 潮州市| 呈贡县| 宁陵县| 阳西县| 军事| 余庆县| 平定县| 广昌县| 建阳市| 乐昌市| 常州市| 拉孜县| 宜州市| 成武县| 清苑县| 禹城市| 柘荣县| 江门市| 乌鲁木齐市| 北流市| 吉首市| 绥棱县| 那曲县| 多伦县| 加查县| 宿州市| 岱山县| 昌黎县| 鹿泉市| 和田市| 京山县| 城市|