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人回腸脂肪酸結(jié)合蛋白(FABP6)酶聯(lián)免疫吸附檢測試劑盒

ELK1536

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human FABP6(Fatty Acid Binding Protein 6, Ileal) ELISA Kit
Reactivity:	Human
Alternative Names:	GT; iBABP; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ILLBP; I-15P; Gastrotropin; Illeal Lipid-Binding Protein; Ileal Bile Acid Binding Protein; Intestinal 15 kDa protein
Assay Type:	Sandwich
Sensitivity:	26.9 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;
Uniprot ID:	P51161
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FABP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FABP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FABP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FABP6 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	2.108	2.010
2000.00	1.647	1.549
1000.00	1.135	1.037
500.00	0.886	0.788
250.00	0.543	0.445
125.00	0.321	0.223
62.50	0.225	0.127
0.00	0.098	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant FABP6 and the recovery rates were calculated by comparing the measured value to the expected amount of FABP6 in samples.

Matrix	Recovery range	Average
serum(n=5)	94-105%	98%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	80-93%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FABP6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.