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大鼠腎素(REN)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1651

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Rat REN(Renin) ELISA Kit
Reactivity:	Rat
Alternative Names:	angiotensinogenase
Assay Type:	Sandwich
Sensitivity:	5.7 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism;
Uniprot ID:	P08424
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REN in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.096	2.013
500.00	1.602	1.519
250.00	1.144	1.061
125.00	0.933	0.850
62.50	0.548	0.465
31.25	0.305	0.222
15.63	0.235	0.152
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant REN and the recovery rates were calculated by comparing the measured value to the expected amount of REN in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	87-95%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of REN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.