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人血小板反應(yīng)蛋白1(TSP-1)酶聯(lián)免疫吸附檢測試劑盒

ELK1685

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human TSP-1(Thrombospondin-1) ELISA Kit
Reactivity:	Human
Alternative Names:	TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type:	Sandwich
Sensitivity:	53 pg/mL
Standard:	8000 pg/mL
Detection Range:	125-8000 pg/mL
Sample Type:	plasma
Assay Length:	3.5h
Research Area:	Infection immunity;Hematology;
Uniprot ID:	P07996
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
8000.00	2.164	2.040
4000.00	1.782	1.658
2000.00	1.205	1.081
1000.00	0.963	0.839
500.00	0.547	0.423
250.00	0.339	0.215
125.00	0.227	0.103
0.00	0.124	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.