產(chǎn)品中心

大鼠血小板反應蛋白1(TSP-1)酶聯(lián)免疫吸附檢測試劑盒

ELK1686

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Rat TSP-1(Thrombospondin-1) ELISA Kit
Reactivity:	Rat
Alternative Names:	TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type:	Sandwich
Sensitivity:	3.6 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Plasma
Assay Length:	3.5h
Research Area:	Infection immunity;Hematology;
Uniprot ID:	Q71SA3
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.368	2.285
250.00	1.720	1.637
125.00	1.225	1.142
62.50	0.782	0.699
31.25	0.586	0.503
15.63	0.321	0.238
7.82	0.251	0.168
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	92-105%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.