免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

小鼠胱天蛋白酶9(CASP9)酶聯(lián)免疫吸附檢測試劑盒
ELK1702
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Mouse CASP9(Caspase 9) ELISA Kit
Reactivity: Mouse
Alternative Names: APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6
Assay Type: Sandwich
Sensitivity: 0.073 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;
Uniprot ID: Q8C3Q9
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CASP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CASP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CASP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL) OD Corrected OD
10.00 2.075 1.994
5.00 1.593 1.512
2.50 1.115 1.034
1.25 0.933 0.852
0.63 0.468 0.387
0.32 0.315 0.234
0.16 0.226 0.145
0.00 0.081 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CASP9 and the recovery rates were calculated by comparing the measured value to the expected amount of CASP9 in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 90-105% 97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CASP9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-107% 84-98% 95-103% 86-98%
EDTA plasma(n=5) 86-93% 91-105% 87-98% 89-94%
Heparin plasma(n=5) 89-99% 83-101% 88-96% 90-101%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
沽源县| 偃师市| 吴旗县| 遂川县| 兴隆县| 佛坪县| 西青区| 苍梧县| 永泰县| 卓资县| 彰化县| 津南区| 普兰县| 扬中市| 怀安县| 射阳县| 河南省| 仲巴县| 遵义县| 于田县| 河曲县| 那坡县| 邮箱| 北辰区| 佛学| 广昌县| 肃北| 咸丰县| 荆州市| 福清市| 盘锦市| 鞍山市| 东兰县| 邢台市| 江陵县| 越西县| 广灵县| 方正县| 枣强县| 正蓝旗| 遂川县|