免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

人谷胱甘肽S轉(zhuǎn)移酶α5(GSTa5)酶聯(lián)免疫吸附檢測試劑盒
ELK1716
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Human GSTa5(Glutathione S Transferase Alpha 5) ELISA Kit
Reactivity: Human
Alternative Names: GST-a5; Glutathione S-Transferase A5; GST class-alpha member 5
Assay Type: Sandwich
Sensitivity: 0.238 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Hepatology;
Uniprot ID: Q7RTV2
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSTa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSTa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSTa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSTa5 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL) OD Corrected OD
40.00 2.126 2.037
20.00 1.536 1.447
10.00 1.196 1.107
5.00 0.852 0.763
2.50 0.554 0.465
1.25 0.372 0.283
0.63 0.175 0.086
0.00 0.089 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GSTa5 and the recovery rates were calculated by comparing the measured value to the expected amount of GSTa5 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 86-99% 92%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSTa5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 79-96% 92-105% 91-99%
EDTA plasma(n=5) 88-105% 79-93% 87-98% 85-96%
Heparin plasma(n=5) 88-97% 97-105% 81-93% 89-102%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
化州市| 韩城市| 隆昌县| 桐梓县| 河间市| 怀来县| 苏尼特左旗| 鄂伦春自治旗| 顺昌县| 靖州| 塔河县| 麟游县| 桃源县| 遂宁市| 高台县| 小金县| 依安县| 大石桥市| 卓资县| 封丘县| 正定县| 奈曼旗| 绿春县| 怀化市| 濮阳市| 饶河县| 武城县| 昌吉市| 秦皇岛市| 巴彦淖尔市| 扎兰屯市| 江津市| 西乌珠穆沁旗| 霍城县| 嘉禾县| 新巴尔虎左旗| 鱼台县| 大余县| 满洲里市| 德惠市| 永靖县|