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大鼠氨基己糖苷酶Bβ(HEXb)酶聯(lián)免疫吸附檢測試劑盒

ELK1718

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Rat HEXb(Hexosaminidase B Beta) ELISA Kit
Reactivity:	Rat
Alternative Names:	Hex-B; ENC-1AS; HCC-7; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta
Assay Type:	Sandwich
Sensitivity:	0.56 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Neuro science;
Uniprot ID:	Q6AXR4
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HEXb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HEXb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HEXb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HEXb in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.136	2.041
50.00	1.593	1.498
25.00	1.183	1.088
12.50	0.882	0.787
6.25	0.553	0.458
3.13	0.315	0.220
1.57	0.226	0.131
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HEXb and the recovery rates were calculated by comparing the measured value to the expected amount of HEXb in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-93%	85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HEXb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.