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小鼠胰蛋白酶原激活肽(TAP)酶聯(lián)免疫吸附檢測試劑盒

ELK1724

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Mouse TAP(Trypsinogen Activation Peptide) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Trypsinogen Activation Peptide
Assay Type:	Competitive Inhibition
Sensitivity:	45.2 pg/mL
Standard:	10000 pg/mL
Detection Range:	156.25-10000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Infection immunity;Immune molecule;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TAP in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
10000.00	0.197
5000.00	0.332
2500.00	0.567
1250.00	0.878
625.00	1.112
312.50	1.537
156.25	1.715
0.00	2.112

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TAP and the recovery rates were calculated by comparing the measured value to the expected amount of TAP in samples.

Matrix	Recovery range	Average
serum(n=5)	78-95%	86%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	95-107%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.