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人膽囊收縮素八肽(CCK8)酶聯(lián)免疫吸附檢測試劑盒
ELK1745
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Human CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit
Reactivity: Human
Alternative Names: Sincalide
Assay Type: Competitive Inhibition
Sensitivity: 4.46 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCK8 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL) OD Corrected OD
1000.00 0.197
500.00 0.385
250.00 0.575
125.00 0.823
62.50 1.157
31.25 1.592
15.63 1.793
0.00 2.213

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CCK8 and the recovery rates were calculated by comparing the measured value to the expected amount of CCK8 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 92-107% 99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCK8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 97-106% 92-101% 82-90%
EDTA plasma(n=5) 85-92% 97-103% 87-98% 95-102%
Heparin plasma(n=5) 85-104% 79-92% 83-95% 86-97%
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