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人低分子量激肽原(LMWK)酶聯(lián)免疫吸附檢測試劑盒

ELK1799

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human LMWK(Low Molecular Weight Kininogen) ELISA Kit
Reactivity:	Human
Alternative Names:	Low Molecular Weight Kininogen
Assay Type:	Sandwich
Sensitivity:	2.99 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample Type:	Plasma.
Assay Length:	3.5h
Research Area:	Signal transduction;Metabolic pathway;Hematology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LMWK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LMWK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LMWK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LMWK in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
400.00	2.179	2.102
200.00	1.612	1.535
100.00	1.116	1.039
50.00	0.831	0.754
25.00	0.591	0.514
12.50	0.356	0.279
6.25	0.189	0.112
0.00	0.077	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LMWK and the recovery rates were calculated by comparing the measured value to the expected amount of LMWK in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-97%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LMWK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.