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人神經(jīng)降壓素(NT)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1802

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human NT(Neurotensin) ELISA Kit
Reactivity:	Human
Alternative Names:	NTS; NTS1; NmN-125; NN; Tail peptide Neuromedin N
Assay Type:	Competitive Inhibition
Sensitivity:	14.2 pg/mL
Standard:	3000 pg/mL
Detection Range:	46.88-3000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Reproductive science;Neuro science;Hormone metabolism;
Uniprot ID:	P30990
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
3000.00	0.235
1500.00	0.389
750.00	0.655
375.00	0.856
187.50	1.225
93.75	1.596
46.88	1.761
0.00	2.325

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NT and the recovery rates were calculated by comparing the measured value to the expected amount of NT in samples.

Matrix	Recovery range	Average
serum(n=5)	95-108%	101%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	80-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.