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人胎盤堿性磷酸酶(ALPP)酶聯(lián)免疫吸附檢測試劑盒
ELK1810
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview

Product name: Human ALPP(Alkaline Phosphatase, Placental) ELISA Kit
Reactivity: Human
Alternative Names: PLAP1; ALP; ALPG; ALPPL; GCAP; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1
Assay Type: Sandwich
Sensitivity: 50 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;
Uniprot ID: P05187
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALPP in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL) OD Corrected OD
8000.00 2.036 1.947
4000.00 1.682 1.593
2000.00 1.107 1.018
1000.00 0.821 0.732
500.00 0.538 0.449
250.00 0.322 0.233
125.00 0.196 0.107
0.00 0.089 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ALPP and the recovery rates were calculated by comparing the measured value to the expected amount of ALPP in samples.
Matrix Recovery range Average
serum(n=5) 89-103% 96%
EDTA plasma(n=5) 78-93% 85%
Heparin plasma(n=5) 82-95% 88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALPP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 93-101% 81-89% 85-99%
EDTA plasma(n=5) 91-103% 86-92% 88-97% 89-97%
Heparin plasma(n=5) 79-94% 87-96% 78-93% 82-90%
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