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人谷胱甘肽還原酶(GR)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1877

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Human GR(Glutathione Reductase) ELISA Kit
Reactivity:	Human
Alternative Names:	GSR; GLUR; GRD1; Glutathione reductase, mitochondrial
Assay Type:	Sandwich
Sensitivity:	34 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Metabolic pathway;
Uniprot ID:	P00390
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GR in the samples is then determined by comparing the OD of the samples to the standard curve.

Environmental Research

Evaluation of genotoxicity, oxidative stress and immune parameters of auto-paint workers

日期：2024-04-28 IF：8.3

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.842	1.759
2500.00	1.587	1.504
1250.00	1.196	1.113
625.00	0.826	0.743
312.50	0.457	0.374
156.25	0.329	0.246
78.13	0.291	0.208
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GR and the recovery rates were calculated by comparing the measured value to the expected amount of GR in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	93-107%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.