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人毛細(xì)血管擴(kuò)張性共濟(jì)失調(diào)突變因子(ATM)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1896

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human ATM(Ataxia Telangiectasia Mutated) ELISA Kit
Reactivity:	Human
Alternative Names:	AT1; ATD; ATA; ATC; ATDC; ATE; TEL1; TELO1; Serine-protein kinase ATM
Assay Type:	Sandwich
Sensitivity:	6.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Tumor immunity;Neuro science;
Uniprot ID:	Q13315
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATM in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.941	1.856
500.00	1.625	1.540
250.00	1.116	1.031
125.00	0.831	0.746
62.50	0.591	0.506
31.25	0.363	0.278
15.63	0.258	0.173
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ATM and the recovery rates were calculated by comparing the measured value to the expected amount of ATM in samples.

Matrix	Recovery range	Average
serum(n=5)	94-107%	100%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.