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人α-黑素細(xì)胞刺激素(aMSH)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK1924

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human aMSH(Alpha-Melanocyte Stimulating Hormone) ELISA Kit
Reactivity:	Human
Alternative Names:	α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin
Assay Type:	Competitive Inhibition
Sensitivity:	45 pg/mL
Standard:	10000 pg/mL
Detection Range:	156.25-10000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Tumor immunity;Endocrinology;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aMSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aMSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aMSH in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
10000.00	0.175
5000.00	0.367
2500.00	0.557
1250.00	0.861
625.00	1.152
312.50	1.511
156.25	1.799
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant aMSH and the recovery rates were calculated by comparing the measured value to the expected amount of aMSH in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	87-99%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of aMSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.