免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

大鼠促甲狀腺素(TSH)酶聯(lián)免疫吸附檢測試劑盒
ELK2283
規(guī)格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview 文獻(xiàn)

Product name: Rat TSH(Thyroid Stimulating Hormone) ELISA Kit
Reactivity: Rat
Alternative Names: Thyrotropin
Assay Type: Sandwich
Sensitivity: 0.071 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSH in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL) OD Corrected OD
10.00 2.054 1.941
5.00 1.687 1.574
2.50 1.142 1.029
1.25 0.855 0.742
0.63 0.576 0.463
0.32 0.389 0.276
0.16 0.235 0.122
0.00 0.113 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TSH and the recovery rates were calculated by comparing the measured value to the expected amount of TSH in samples.
Matrix Recovery range Average
serum(n=5) 80-98% 89%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 84-98% 91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 88-96% 77-91% 85-93%
EDTA plasma(n=5) 87-102% 96-105% 85-93% 89-97%
Heparin plasma(n=5) 95-104% 87-96% 88-101% 82-95%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
娄底市| 乐山市| 绥宁县| 句容市| 开远市| 乌兰察布市| 色达县| 岚皋县| 达尔| 南召县| 肥东县| 来安县| 库车县| 沁源县| 隆昌县| 泗洪县| 抚松县| 宁陵县| 来凤县| 安溪县| 高陵县| 滨海县| 云霄县| 瑞昌市| 临沂市| 苍南县| 同德县| 额尔古纳市| 涟水县| 阿拉善右旗| 尉氏县| 茶陵县| 革吉县| 海阳市| 左云县| 宝清县| 临桂县| 沙河市| 乃东县| 七台河市| 沭阳县|