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人骨鈣素(OC)酶聯(lián)免疫吸附檢測試劑盒

ELK2390

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human OC(Osteocalcin) ELISA Kit
Reactivity:	Human
Alternative Names:	BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Assay Type:	Sandwich
Sensitivity:	0.252 ng/mL
Standard:	40 ng/mL
Detection Range:	0.63-40 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Uniprot ID:	P02818
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.

PeerJ

Changes in the concentration of bone turnover markers in men after maximum intensity exercise

日期：2024-05-17 IF：2.7
CELLULAR AND MOLECULAR BIOLOGY

Evaluation of temporomandibular joint morphology and morphometry in male osteoporotic patients using advanced imaging and biochemical markers: A cross-sectional study

日期：2024-06-05 IF：1.5

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
40.00	2.079	1.996
20.00	1.556	1.473
10.00	1.174	1.091
5.00	0.963	0.880
2.50	0.572	0.489
1.25	0.312	0.229
0.63	0.156	0.073
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OC and the recovery rates were calculated by comparing the measured value to the expected amount of OC in samples.

Matrix	Recovery range	Average
serum(n=5)	80-94%	87%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.