91看片淫黄大片一级在线观看_人全段甲狀旁腺素(I-PTH)酶聯(lián)免疫吸附檢測試劑盒-科鹿（武漢）生物科技有限責任公司

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人全段甲狀旁腺素(I-PTH)酶聯(lián)免疫吸附檢測試劑盒

ELK2427

規格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Human I-PTH(IntactParathormone) ELISA Kit
Reactivity:	Human
Alternative Names:	iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type:	Sandwich
Sensitivity:	4.95 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Diabetology & Metabolic Syndrome

Association between bone turnover markers, bone mineral density, and serum osteoglycine in middle-aged men with Type 2 Diabetes mellitus

日期：2024-07-09 IF：3.4

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.989	1.900
500.00	1.633	1.544
250.00	1.121	1.032
125.00	0.726	0.637
62.50	0.529	0.440
31.25	0.322	0.233
15.63	0.252	0.163
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-95%	89%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.