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小鼠金屬硫蛋白1(MT1)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK2455

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Mouse MT1(Metallothionein 1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	MT1A; MT1S; MTC
Assay Type:	Sandwich
Sensitivity:	26.3 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Tumor immunity;
Uniprot ID:	P02802
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

MUSCLE & NERVE

Amyotrophic Lateral Sclerosis and swim training affect copper metabolism in skeletal muscle in a mouse model of disease

日期：2024-09-03 IF：2.8

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.983	1.894
2000.00	1.638	1.549
1000.00	1.133	1.044
500.00	0.877	0.788
250.00	0.496	0.407
125.00	0.312	0.223
62.50	0.206	0.117
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MT1 and the recovery rates were calculated by comparing the measured value to the expected amount of MT1 in samples.

Matrix	Recovery range	Average
serum(n=5)	93-105%	99%
EDTA plasma(n=5)	84-93%	89%
Heparin plasma(n=5)	81-103%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.