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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
100.00 | 2.126 | 2.039 |
50.00 | 1.487 | 1.400 |
25.00 | 1.043 | 0.956 |
12.50 | 0.625 | 0.538 |
6.25 | 0.428 | 0.341 |
3.13 | 0.340 | 0.253 |
1.57 | 0.176 | 0.089 |
0.00 | 0.087 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 88-102% | 95% |
EDTA plasma(n=5) | 90-105% | 97% |
Heparin plasma(n=5) | 80-92% | 86% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-97% | 86-92% | 89-103% | 89-98% |
EDTA plasma(n=5) | 90-99% | 95-106% | 87-101% | 82-97% |
Heparin plasma(n=5) | 79-92% | 80-93% | 81-98% | 89-102% |
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