97精品一区二区三区_人骨誘導因子(OGN)酶聯(lián)免疫吸附檢測試劑盒-科鹿（武漢）生物科技有限責任公司

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人骨誘導因子(OGN)酶聯(lián)免疫吸附檢測試劑盒

ELK3335

規格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Human OGN(Osteoglycin) ELISA Kit
Reactivity:	Human
Alternative Names:	OIF; SLRR3A; Mimecan Proteoglycan; Osteoinductive Factor
Assay Type:	Sandwich
Sensitivity:	12.5 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;
Uniprot ID:	P20774
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGN in the samples is then determined by comparing the OD of the samples to the standard curve.

Diabetology & Metabolic Syndrome

Association between bone turnover markers, bone mineral density, and serum osteoglycine in middle-aged men with Type 2 Diabetes mellitus

日期：2024-07-09 IF：3.4

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.266	2.177
1000.00	1.556	1.467
500.00	1.128	1.039
250.00	0.792	0.703
125.00	0.457	0.368
62.50	0.256	0.167
31.25	0.226	0.137
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant OGN and the recovery rates were calculated by comparing the measured value to the expected amount of OGN in samples.

Matrix	Recovery range	Average
serum(n=5)	87-95%	91%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	83-97%	90%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OGN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.