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人心肌肌鈣蛋白T(cTnT/TNNT2)酶聯(lián)免疫吸附檢測(cè)試劑盒
ELK3961 規(guī)格： |
 價(jià)格： |
|---|---|
| 48T | ￥1960.00 |
| 96T | ￥2800.00 |
下載說(shuō)明 ①Overview 文獻(xiàn)
日期：2025-01-13
IF：3.4標(biāo)準(zhǔn)曲線(xiàn)
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 1.925 | 1.838 |
| 500.00 | 1.379 | 1.292 |
| 250.00 | 1.116 | 1.029 |
| 125.00 | 0.782 | 0.695 |
| 62.50 | 0.554 | 0.467 |
| 31.25 | 0.332 | 0.245 |
| 15.63 | 0.226 | 0.139 |
| 0.00 | 0.087 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 83-99% | 95% |
| Heparin plasma(n=5) | 85-97% | 91% |
線(xiàn)性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-102% | 87-102% | 85-96% | 87-101% |
| EDTA plasma(n=5) | 96-103% | 83-96% | 87-98% | 85-97% |
| Heparin plasma(n=5) | 87-96% | 92-105% | 89-102% | 92-101% |
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