產(chǎn)品中心

人載脂蛋白A1結(jié)合蛋白(APOA1BP)酶聯(lián)免疫吸附檢測試劑盒

ELK4941

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Human APOA1BP(Apolipoprotein A1 Binding Protein) ELISA Kit
Reactivity:	Human
Alternative Names:	Apo-A1BP; AIBP; YJEFN1; NAD(P)H-hydrate epimerase; NAD(P)HX epimerase; YjeF N-terminal domain-containing protein 1
Assay Type:	Sandwich
Sensitivity:	0.061 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Metabolic pathway;
Uniprot ID:	Q8NCW5
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOA1BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOA1BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOA1BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOA1BP in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in lmmunology

LC-MS/MS analysis reveals plasma protein signatures associated with lymph node metastasis in colorectal cancer

日期：2024-10-23 IF：0

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.045	1.976
5.00	1.579	1.510
2.50	1.112	1.043
1.25	0.813	0.744
0.63	0.532	0.463
0.32	0.335	0.266
0.16	0.235	0.166
0.00	0.069	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant APOA1BP and the recovery rates were calculated by comparing the measured value to the expected amount of APOA1BP in samples.

Matrix	Recovery range	Average
serum(n=5)	84-98%	91%
EDTA plasma(n=5)	80-96%	88%
Heparin plasma(n=5)	82-98%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOA1BP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.