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豬白介素12A(IL12A)酶聯(lián)免疫吸附檢測試劑盒

ELK5877

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Pig IL12A(Interleukin 12A) ELISA Kit
Reactivity:	Pig
Alternative Names:	IL-12A;p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1
Assay Type:	Sandwich
Sensitivity:	6.4 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Uniprot ID:	Q29053
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IL12A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL12A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IL12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IL12A in the samples is then determined by comparing the OD of the samples to the standard curve.

PAKISTAN VETERINARY JOURNAL

Effects of dietary intake of heat-inactivated Limosilactobacillus reuteri PSC102 on the growth performance, immune response, and gut microbiota in weaned piglets

日期：2024-08-08 IF：3.8

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.043	1.958
500.00	1.527	1.442
250.00	1.035	0.950
125.00	0.797	0.712
62.50	0.543	0.458
31.25	0.351	0.266
15.63	0.226	0.141
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL12A and the recovery rates were calculated by comparing the measured value to the expected amount of IL12A in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	87-97%	90%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.