產(chǎn)品中心

5-羥基吲哚乙酸(5-HIAA)酶聯(lián)免疫吸附檢測試劑盒

ELK7838

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
Reactivity:	General
Alternative Names:	5HIAA
Assay Type:	Competitive Inhibition
Sensitivity:	1.93 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample Type:	serum, plasma, urine, cerebrospinal fluid and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Tumor immunity;Endocrinology;Urology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HIAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HIAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Biology Reports

Role of Thymoquinone on sleep restriction and its mitigating effect on leptin-mediated signaling pathway in rat brain

日期：2024-06-14 IF：2.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
400.00	0.199
200.00	0.352
100.00	0.577
50.00	0.895
25.00	1.139
12.50	1.578
6.25	1.789
0.00	2.115

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 5-HIAA and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HIAA in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	92-105%	93%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HIAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.