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去甲腎上腺素(NE)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK7885

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	NE(Noradrenaline) ELISA Kit
Reactivity:	General
Alternative Names:	NA; NAd; Norepinephrine; Norepi
Assay Type:	Competitive Inhibition
Sensitivity:	26.1 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NE in the samples is then determined by comparing the OD of the samples to the standard curve.

International Journal of Medical Sciences

Left Stellate Ganglion Ablation Inhibits Ventricular Arrhythmias through Macrophage Regulation in Canines with Acute Ischemic Stroke

日期：2021-10-20 IF：2.557
Peptides

C-type natriuretic peptide suppresses ventricular arrhythmias in rats with acute myocardial ischemia

日期：2021-10-21 IF：2.898
International Journal of Medical Sciences

Left Stellate Ganglion Ablation Inhibits Ventricular Arrhythmias through Macrophage Regulation in Canines with Acute Ischemic Stroke

日期：2021-10-26 IF：2.557
Environmental Toxicology

Dapagliflozin Protects Methamphetamine-Induced Cardiomyopathy by Alleviating Mitochondrial Damage and Reducing Cardiac Function Decline in a Mouse Model

日期：2022-07-07 IF：4.109
International Journal of Medical Sciences

Blockade of SK4 channels suppresses atrial fibrillation by attenuating atrial fibrosis in canines with prolonged atrial pacing

日期：2022-11-23 IF：3.642
Small Ruminant Research

Behavioural and neuroendocrine responses in alpacas (Vicugna pacos) to the shearing procedure

日期：2024-10-22 IF：1.6

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
5000.00	0.189
2500.00	0.396
1250.00	0.557
625.00	0.857
312.50	1.152
156.25	1.565
78.13	1.793
0.00	2.226

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.

Matrix	Recovery range	Average
serum(n=5)	82-97%	89%
EDTA plasma(n=5)	84-93%	87%
Heparin plasma(n=5)	95-108%	85%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.