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乙酰肉毒堿(ALCAR)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8184

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	ALCAR(Acetylcarnitine) ELISA Kit
Reactivity:	General
Alternative Names:	Acetyl-L-carnitine
Assay Type:	Competitive Inhibition
Sensitivity:	25.2 ng/mL
Standard:	5000 ng/mL
Detection Range:	78.13-5000 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Metabolic pathway;Neuro science;Nutrition metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ALCAR protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ALCAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ALCAR in the samples is then determined by comparing the OD of the samples to the standard curve.

Biomedical and Biotechnology Research Journal (BBRJ)

Serum Acetylcarnitine as a Diagnostic Marker in Depression Episodes

日期：2024-06-29 IF：

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
5000.00	0.191
2500.00	0.367
1250.00	0.615
625.00	0.833
312.50	1.275
156.25	1.501
78.13	1.793
0.00	2.057

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ALCAR and the recovery rates were calculated by comparing the measured value to the expected amount of ALCAR in samples.

Matrix	Recovery range	Average
serum(n=5)	78-96%	87%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	96-107%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALCAR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.