免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

雞皮質(zhì)醇(Cor)酶聯(lián)免疫吸附檢測(cè)試劑盒
ELK8733
規(guī)格: 價(jià)格:
48T ¥2240.00
96T ¥3200.00

Overview 文獻(xiàn)

Product name: Chicken Cor(Cortisol) ELISA Kit
Reactivity: Chicken
Alternative Names: Hydrocortisone
Assay Type: Competitive Inhibition
Sensitivity: 0.93 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken Cor protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken Cor in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.336
50.00 0.611
25.00 0.913
12.50 1.152
6.25 1.583
3.13 1.789
0.00 2.115

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cor and the recovery rates were calculated by comparing the measured value to the expected amount of Cor in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 78-93% 90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 83-97% 88-95% 96-102%
EDTA plasma(n=5) 85-100% 84-98% 95-101% 88-102%
Heparin plasma(n=5) 85-94% 87-96% 82-95% 92-105%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號(hào)

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
鄂伦春自治旗| 文水县| 石狮市| 临夏县| 贵州省| 建昌县| 合川市| 兰考县| 南郑县| 报价| 博野县| 大英县| 桐柏县| 西宁市| 民乐县| 鹤峰县| 运城市| 专栏| 集安市| 鄂尔多斯市| 东安县| 崇左市| 呼图壁县| 弥渡县| 黄大仙区| 克拉玛依市| 两当县| 安达市| 文山县| 左权县| 花莲市| 独山县| 历史| 吴旗县| 河曲县| 怀来县| 威宁| 读书| 乌鲁木齐县| 大英县| 姜堰市|