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大鼠胱天蛋白酶6(CASP6)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8812

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat CASP6(Caspase 6) ELISA Kit
Reactivity:	Rat
Alternative Names:	MCH2; Cysteinyl Aspartate Specific Proteinases 6; Caspase 6,Apoptosis-Related Cysteine Peptidase; Apoptotic protease Mch-2
Assay Type:	Sandwich
Sensitivity:	0.065 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Apoptosis;
Uniprot ID:	O35397
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Biochemical and Biophysical Research Communications

Acetate mitigates cardiac mitochondrial dysfunction in experimental model of polycystic ovarian syndrome by modulating GPCR41/43 and PROKR1

日期：2023-09-21 IF：3.1
BMC Pharmacology and Toxicology

Butyrate alleviates renal inflammation and fibrosis in a rat model of polycystic ovarian syndrome by suppression of SDF-1

日期：2023-10-03 IF：2.9
BMC Neuroscience

Modulation of GABA by sodium butyrate ameliorates hypothalamic inflammation in experimental model of PCOS

日期：2024-02-28 IF：2.4
Modulation of PON-1 by acetate reverses hepatic dysmetabolism in experimentally induced PCOS

Comparative Clinical Pathology

日期：2024-02-06 IF：3.4
Journal of Physiological Sciences

Acetate ameliorates ovarian mitochondrial dysfunction in letrozole-induced polycystic ovarian syndrome rat model by improving mitofusin-2

日期：2024-04-01 IF：2.3
Nutrire

Butyrate alleviates adipose mitochondrial dysfunction and inflammation in experimental model of polycystic ovarian syndrome by modulating SIRT1-dependent mechanism

日期：2024-06-12 IF：1
Comparative Clinical Pathology

Modulation of PON-1 by acetate reverses hepatic dysmetabolism in experimentally induced PCOS

日期：2024-02-06 IF：1.3
BMC Research Notes

Acetate attenuates hypothalamic pyroptosis in experimentally induced polycystic ovarian syndrome

日期：2024-09-12 IF：1.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.045	1.937
5.00	1.673	1.565
2.50	1.136	1.028
1.25	0.975	0.867
0.63	0.556	0.448
0.32	0.329	0.221
0.16	0.226	0.118
0.00	0.108	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CASP6 and the recovery rates were calculated by comparing the measured value to the expected amount of CASP6 in samples.

Matrix	Recovery range	Average
serum(n=5)	96-107%	102%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	83-95%	89%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CASP6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.