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綿羊胰島素樣生長因子1(IGF1)酶聯(lián)免疫吸附檢測試劑盒

ELK8849

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻

Product name:	Sheep IGF1(insulin-like growth factors 1) ELISA Kit
Reactivity:	Sheep
Alternative Names:	IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
Assay Type:	Sandwich
Sensitivity:	0.69 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Endocrinology;
Uniprot ID:	P10763
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

International Journal of Inflammation

The Effect of Endotoxin-Induced Inflammation on the Activity of the Somatotropic Axis in Sheep

日期：2024-08-07 IF：2.6

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.207	2.111
50.00	1.587	1.491
25.00	1.118	1.022
12.50	0.861	0.765
6.25	0.559	0.463
3.13	0.265	0.169
1.57	0.325	0.229
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IGF1 and the recovery rates were calculated by comparing the measured value to the expected amount of IGF1 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-105%	98%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	80-94%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.