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小鼠睪酮(Testo)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK9029

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

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Overview 文獻(xiàn)

Product name:	Mouse Testo(Testosterone) ELISA Kit
Reactivity:	Mouse
Alternative Names:	T; Testesteron; Testeronun
Assay Type:	Competitive Inhibition
Sensitivity:	17.7 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.5-4000 pg/mL
Sample Type:	serum, plasma, other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

Comparative Clinical Pathology

Ameliorative effects of quercetin on endocrine and metabolic abnormalities associated with experimentally induced polycystic ovary syndrome in mice

日期：2023-02-08 IF：-
Ecotoxicology and Environmental Safety

The male reproductive toxicity after 5-Fluorouracil exposure: DNA damage, oxidative stress, and mitochondrial dysfunction in vitro and in vivo

日期：2024-06-15 IF：6.2
Journal of Ethnopharmacology

The chemometrics analysis and integrated pharmacology approach to decipher the effect and mechanism between raw and processed cistanche tubulosa

日期：2024-06-28 IF：4.8
BMC Endocrine Disorders

Diacerein mitigates endocrine and cardio-metabolic disruptions in experimental PCOS mice model by modulating AdipoR1/ PON 1

日期：2024-07-14 IF：2.8

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	0.183
2000.00	0.367
1000.00	0.611
500.00	0.812
250.00	1.139
125.00	1.596
62.50	1.863
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Testo and the recovery rates were calculated by comparing the measured value to the expected amount of Testo in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	82-98%	90%
Heparin plasma(n=5)	95-107%	85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testo and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.