免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

豬皮質(zhì)醇(Cor)酶聯(lián)免疫吸附檢測試劑盒
ELK9064
規(guī)格: 價格:
48T ¥2240.00
96T ¥3200.00

Overview 文獻(xiàn)

Product name: Pig Cor(Cortisol) ELISA Kit
Reactivity: Pig
Alternative Names: Hydrocortisone
Assay Type: Competitive Inhibition
Sensitivity: 0.93 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig Cor protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Cor in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.311
50.00 0.527
25.00 0.856
12.50 1.152
6.25 1.557
3.13 1.697
0.00 2.219

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cor and the recovery rates were calculated by comparing the measured value to the expected amount of Cor in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 88-102% 97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 97-106% 85-93% 78-97%
EDTA plasma(n=5) 95-102% 89-96% 87-98% 85-93%
Heparin plasma(n=5) 87-98% 87-102% 86-93% 91-105%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
普安县| 淳安县| 河北区| 米脂县| 秦皇岛市| 合作市| 财经| 萨迦县| 阿克苏市| 凤翔县| 宁安市| 鹤壁市| 逊克县| 聂荣县| 恩施市| 洛隆县| 桐庐县| 固始县| 永川市| 封丘县| 会理县| 米易县| 涟水县| 虎林市| 泰安市| 吴桥县| 栾川县| 黄梅县| 滨海县| 吉木乃县| 阳西县| 黄梅县| 长治市| 隆子县| 龙游县| 景德镇市| 封丘县| 石河子市| 修水县| 山阳县| 潼南县|